In vitro exposure to triazoles used as fungicides impairs human granulosa cells steroidogenesis - BRGM - Bureau de recherches géologiques et minières Access content directly
Journal Articles Environmental Toxicology and Pharmacology Year : 2023

In vitro exposure to triazoles used as fungicides impairs human granulosa cells steroidogenesis

Abstract

Triazoles are the main components of fungicides used in conventional agriculture. Some data suggests that they may be endocrine disruptors. Here, we found five triazoles, prothioconazole, metconazole, difenoconazole, tetraconazole, and cyproconazole, in soil or water from the Centre-Val de Loire region of France. We then studied their effects from 0.001 µM to 1000 µM for 48 h on the steroidogenesis and cytotoxicity of ovarian cells from patients in this region and the human granulosa line KGN. In addition, the expression of the aryl hydrocarbon receptor (AHR) nuclear receptor in KGN cells was studied. Overall, all triazoles reduced the secretion of progesterone, estradiol, or both at doses that were non-cytotoxic but higher than those found in the environment. This was mainly associated, depending on the triazole, with a decrease in the expression of CYP51, STAR, CYP11A1, CYP19A1, or HSD3B proteins, or a combination thereof, in hGCs and KGN cells and an increase in AHR in KGN cells.

Domains

Earth Sciences
Fichier principal
Vignette du fichier
1-s2.0-S1382668923002375-main.pdf (1.97 Mo) Télécharger le fichier
Origin : Publication funded by an institution

Dates and versions

hal-04314240 , version 1 (29-11-2023)

Licence

Attribution

Identifiers

Cite

Loïse Serra, Anthony Estienne, Giovanni Caria, Christelle Ramé, Claudy Jolivet, et al.. In vitro exposure to triazoles used as fungicides impairs human granulosa cells steroidogenesis. Environmental Toxicology and Pharmacology, 2023, 104, pp.104295. ⟨10.1016/j.etap.2023.104295⟩. ⟨hal-04314240⟩
33 View
18 Download

Altmetric

Share

Gmail Facebook X LinkedIn More