Development of molecular assays to efficiently monitor and quantify microbial species during bioleaching of copper-rich ores - BRGM - Bureau de recherches géologiques et minières Access content directly
Conference Papers Year : 2016

Development of molecular assays to efficiently monitor and quantify microbial species during bioleaching of copper-rich ores

Abstract

Monitoring of the microbial community in bioleaching processes is essential in order to control process parameters and enhance the leaching efficiency. Suitable methods are, however, limited as nucleic acid extraction is sometimes inefficient and often no specific assays are described for these types of consortia. Therefore we have developed reliable assays to monitor a specific microbial consortium used for bioleaching of copper-rich ores. Our investigations comprised the efficient recovery of nucleic acids from bioleaching reactors and subsequent quantification of the microorganisms at species level via T-RFLP and qPCR. Therefore we developed novel qPCR assays specific for bioleaching species. The qPCR assays were validated in two different labs and also successfully applied to monitor various bioleaching experiments. Furthermore, a pre-treatment method for efficient fluorescence staining of whole cells from these reactors was developed and approved. These novel assays can be applied to various bioleaching operations for fast and efficient community monitoring.
Fichier principal
Vignette du fichier
Hedrich et al_Biohydromet 16.pdf (7.07 Ko) Télécharger le fichier
Origin Files produced by the author(s)

Dates and versions

hal-01309327 , version 1 (29-04-2016)

Identifiers

  • HAL Id : hal-01309327 , version 1

Cite

Sabrina Hedrich, Anne-Gwenaelle Guezennec, Mickaël Charron, Axel Schippers, Catherine Joulian. Development of molecular assays to efficiently monitor and quantify microbial species during bioleaching of copper-rich ores. Biohydromet'16, Jun 2016, Falmouth, United Kingdom. ⟨hal-01309327⟩

Collections

BRGM ANR BRGM-DO
158 View
50 Download

Share

Gmail Mastodon Facebook X LinkedIn More