The contribution of human activities such as industries, agriculture and domestic inputs, becomes more and more significant in the chemical composition of the dissolved load of rivers. Human factors act as a supplementary key process. Therefore the mass-balance for the budget of catchments and river basins include anthropogenic disturbances. The Loire River in central France is approximately 1010 km long and drains an area of 117,800 km2. In the upper basin, the bedrock is old plutonic rock overlain by much younger volcanic rocks. The intermediate basin includes three major tributaries flowing into the Loire River from the left bank: the Cher, the Indre and the Vienne rivers; the main stream flows westward and its valley stretches toward the Atlantic Ocean. Here, the Loire River drains the sedimentary series of the Paris Basin, mainly carbonate deposits. The lower Loire basin drains pre-Mesozoic basement of the Armorican Massif and its overlying Mesozoic to Cenozoic sedimentary deposits. The Loire River is one of the main European riverine inputs to the Atlantic ocean. Here we are reporting concentration and isotope data for Zn in river waters and suspended sediments from the Loire River Basin. In addition, we also report concentration and isotope data for the different industrial sources within the Loire Basin, as well as data for biota samples such as mussels and oysters from the Bay of Biscay and North Brittany. These organisms are known to be natural accumulators of metal pollutants. Zinc isotopic compositions are rather homogeneous in river waters with δ66Zn values ranging from 0.21 to 0.39‰. This range of variation is very different from anthropogenic signature (industrial and/or agriculture release) that displays δ66Zn values between 0.02 to 0.14‰. This result is in agreement with a geogenic origin and the low Zn concentrations in the Loire River Basin (from 0.8 to 6 µg/L).