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Journal Articles ACS Central Science Year : 2023

Diffusive Dynamics of Bacterial Proteome as a Proxy of Cell Death

Daniele Di Bari
Stepan Timr
Tilo Seydel
Caterina Petrillo
Fabio Sterpone
Alessandro Paciaroni

Abstract

Temperature variations have a big impact on bacterial metabolism and death, yet an exhaustive molecular picture of these processes is still missing. For instance, whether thermal death is determined by the deterioration of the whole or a specific part of the proteome is hotly debated. Here, by monitoring the proteome dynamics of E. coli, we clearly show that only a minor fraction of the proteome unfolds at the cell death. First, we prove that the dynamical state of the E. coli proteome is an excellent proxy for temperature-dependent bacterial metabolism and death. The proteome diffusive dynamics peaks at about the bacterial optimal growth temperature, then a dramatic dynamical slowdown is observed that starts just below the cell’s death temperature. Next, we show that this slowdown is caused by the unfolding of just a small fraction of proteins that establish an entangling interprotein network, dominated by hydrophobic interactions, across the cytoplasm. Finally, the deduced progress of the proteome unfolding and its diffusive dynamics are both key to correctly reproduce the E. coli growth rate.
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hal-03936222 , version 1 (18-01-2023)

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Daniele Di Bari, Stepan Timr, Marianne Guiral, Marie-Thérèse Giudici-Orticoni, Tilo Seydel, et al.. Diffusive Dynamics of Bacterial Proteome as a Proxy of Cell Death. ACS Central Science, 2023, ⟨10.1021/acscentsci.2c01078⟩. ⟨hal-03936222⟩
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